Development of green production technology and research of harpagophytum procumbens root dry extract

The aim of this work is to study standardization parameters and pharmacological properties Harpagophytum procumbens (H. procumbens) root dry extract.
 Materials methods – obtaining H. extract was performed in accordance with developed scheme that characterized by simplicity cost-effectiveness technology compared analogous scheme. Study obtained the requirements monograph «Harpagophyti extractum siccum» State Pharmacopoeia Ukraine 2.2., harmonized European 9.5. Pharmacology analgesic anti-inflammatory activities were conducted on rat paw formalin-induced edema model.
 Results. Obtained a free flowing, non-hygroscopic, light brown powder taste odor specific raw material procumbens. Determined loss drying 3.2±0.18 %. Content heavy metals not more than 0.01 % (100 ppm). Harpagoside identified TLC method. Chromatographic zones chromatograms reference solution test solutions are same intensity color, cross-over sharpness development. Based results research HPLC method, it has been established content harpagoside investigated 2.50±0.02 %.
 According found (the less 37.0 mg/kg) showed higher activity drug («Phong Te Thap»).
 Conclusions. A new method (3.5:1) proposed. green production simple; does require special equipment expensive solvents. correspondence quality Pharmacopoeia: appearance, identification fructose, drying, metals, assay (2.50±0.02 %) set. Analgesic